講演者：Prof. Benjamin Hatton (トロント大学)
タイトル：Designing surfaces to influence microbial adhesion and growth
Microbial attachment to material surfaces is a complex phenomenon involving a complexity of electrostatic, biochemical and physical mechanisms. Despite the major problems associated with medical device-associated infection, infectious disease transmission within healthcare environments, and hospital acquired infections (HAIs), many aspects of microbial surface attachment are not well understood. Rates of HAI remain relatively high, and there is significant concern about the effects of antibiotic resistance, globally. Most approaches to antimicrobial material design still usually just rely on the release of biocidal chemical species (such as Ag ions).
We are interested in how material surface design – factors such as surface chemistry, biochemical functionalization, nano- and microtopography, and wettability – can influence microbial surface attachment and biofilm development. In particular, our work has focussed on strategies to disrupt the early stages of microbial surface attachment in antimicrobial material design, as an alternative to biocidal chemistries. In the context of medical device-associated infections, there is an important advantage to keeping bacteria in a planktonic (swimming) state, as they are much more vulnerable than in a biofilm community.
This talk will summarize several experimental research projects that explore aspects of bacterial cell attachment to material surfaces; (1) The immobilization of glycoside hydrolase enzymes to disrupt pseudomonas aeruginosa biofilms; (2) Nanotopographies in wetting contact (the ‘cicada effect’) ; (3) Nano- and microtopographies in non-wetting (superhydrophobic) contact ; (4) Microbial attraction to topographical surface defects ; and (5) ‘Slippery’ surfaces (SLIPS) in the design of non-specific, non-fouling material. Overall, the application of these various materials chemistry design tools has enabled us to learn something about microbial behaviour, through material surface design.